Dietary docosahexaenoic acid suppresses T cell protein kinase C theta lipid raft recruitment and IL-2 production.

Dietary Docosahexaenoic Acid Suppresses T Cell Protein Kinase C Lipid Raft Recruitment and IL-2 Production

Docosahexaenoic acid changes lipid composition and interleukin-2 receptor signaling in membrane rafts.

Polyunsaturated fatty acids, including docosahexaenoic acid (DHA, 22:6n-3), modulate immune responses and exert beneficial immunosuppressive effects, but the molecular mechanisms inhibiting T-cell activation are not yet elucidated. Lipid rafts have been shown to play an important role in the compartmentalization and modulation of cell signaling. We investigated the role of DHA in modulating the...

Docosahexaenoic Acid Changes Lipid Composition and IL-2 Receptor Signaling in Membrane Rafts Running Title: DHA changes Rafts

Polyunsaturated fatty acids (PUFAs) including docosahexaenoic acid (DHA; 22:6 n-3) modulate immune responses and exert beneficial immunosuppressive effects, but the molecular mechanisms inhibiting T cell activation are not yet elucidated. Lipid rafts have been shown to play an important role in the compartmentalization and modulation of cell signaling. We investigate the role of DHA in modulati...

The Molecular Adapter Carma1 Controls Entry of IκB Kinase into the Central Immune Synapse

Carma1 (also known as caspase recruitment domain [CARD]11, Bimp3) is a CARD-containing membrane-associated guanylate kinase family protein that plays an essential role in antigen receptor-induced nuclear factor kappaB activation. We investigated the role of Carma1 in the assembly of signaling molecules at the immune synapse using a peptide-specific system. We report that Carma1 is essential for...

A molecular framework for two-step T cell signaling: Lck Src homology 3 mutations discriminate distinctly regulated lipid raft reorganization events.

Costimulation by CD28 or lipid-raft-associated CD48 potentiate TCR-induced signals, cytoskeletal reorganization, and IL-2 production. We and others have proposed that costimulators function to construct a raft-based platform(s) especially suited for TCR engagement and sustained and processive signal transduction. Here, we characterize TCR/CD48 and TCR/CD28 costimulation in T cells expressing Lc...